17-Title: Protective effect of quail egg yolk on sperm characteristics of Barbari buck semen during short-term storage at 4 ºC

Authors: Mukul Anand, Sarvajeet Yadav, Brijesh Yadav, Rahul Dhariya and Deependra Chaudhary

Source: Ruminant Science (2023)-12(1):95-98.

How to cite this manuscript: Anand Mukul, Yadav Sarvajeet, Yadav Brijesh, Dhariya Rahul and Chaudhary Deependra (2023). Protective effect of quail egg yolk on sperm characteristics of Barbari buck semen during short-term storage at 4 ºC. Ruminant Science 12(1):95-98.

Abstract

In this experiment, different concentrations of quail egg yolk in semen extender of buck were evaluated for their capacity to protect sperm from cold damage during its short-term storage at 4 ºC. Four Barbari bucks maintained at the goat unit were selected for the experiment. Twelve ejaculates were collected from each buck, with a collection frequency of 2 ejaculates per week. Ejaculates of all bucks were collected on the same day and later evaluated and pooled. Each pooled sample was divided in 5 equal aliquots and diluted with a Tris-egg yolk-glycerol-based extender. Sample supplemented with 3% hen egg yolk in the extender was marked as control (C) while the samples diluted with variable concentrations of quail egg yolk in the semen extender were marked as treatment groups’ viz. T1 (2.5%), T2 (10%), T3 (15%) and T4 (20%). Diluted semen was extended to reach a final concentration of 200 x 106 spermatozoa/ml. The diluted sample was stored at 4°C for 48 hours and later evaluated for viability, motility, acrosomal integrity, hypo-osmotic swelling test, path velocity and kinematic characters. Significantly (p<0.01) higher values of per cent live sperm were observed in T2 and T3 while sperm exhibiting progressive motion, per cent uncapacitated sperm, HOST positive sperm and consistency of sperm membrane were significantly (p<0.01) higher in T2 as compared to other groups evaluated during the experiment. Evaluation of motion characteristics exhibited significantly (p<0.01) higher values for curvilinear velocity (VCL, µm / sec), Beat cross frequency (BCF, hz), amplitude of lateral head displacement (ALH, µm) in T2 and T3 while other kinematic characters average path velocity (VAP, µm/sec), straight-line velocity (VSL, µm/sec), Linearity (LIN, %) and Straightness (STR, %) exhibited by sperm were significantly (p<0.01) higher in T3 as compared to other treatments and control. It may be concluded that 10% quail egg yolk in extender has a better capacity to protect sperm against cold damage and can be an alternate to hen egg yolk for short-term storage of buck semen at 4 °C.

References

Alahmar AT (2019). Role of oxidative stress in male infertility: An updated review. Journal of Human Reproduction Science 12(1):4-18.

Anand M and Yadav S (2016). Assessment of motion and kinematic characteristics of frozen-thawed Sirohi goat semen using computer-assisted semen analysis. Veterinary World 9(2):203-206.

Anand M, Yadav S, Kumar J and Yadav B (2016). Effect of different egg yolk concentration on chilled barbari buck semen during short term storage. Journal of Animal Research 6(4):635-639

Anand M, Bagnel G and Yadav S (2017). Effect of egg yolk concentration and washing on sperm quality following cryopreservation in Barbari buck semen. Journal of Applied Animal Research 45(1):560-565.

Armon L and Eisenbach M (2011). Behavioral mechanism during human sperm chemotaxis: involvement of hyperactivation. PLoS One 6(12): e28359.

Citil OB, Yildirim I and Parlat SS (2011). The effects of dietary flaxseed oil supplementations on fatty acids composition of the yolks in quail (Coturnix coturnix japonica) eggs. Journal of Faculty Veterinary Medicine 17(5):761-766.

Daly J, Smith H, McGrice HA, Kind KL and van Wettere WHEJ (2020). Towards improving the outcomes of assisted reproductive technologies of cattle and sheep, with particular focus on recipient management. Animals 10(2):293-299.

Derdour Salima-Yamina, Mimoune Nora, Laamari Abdelouahab, Saboundji Imad Eddine, Hamadouche Abdelkadir, Khelef Djamel and Kaidi Rachid (2022). Study on factors influencing the success of AI according to the inseminators. Ruminant Science 11(1):21-24.

Hancock JL (1952). The morphology of bull spermatozoa. Journal of Experimental Biology 29:445-453.

Jeyendran RS, Vander-Ven HH, Perez-Pelaez M, Crabo BG and Zanevld LJD (1984) Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characters. Journal of Reproduction and Fertility 70:219-228.

Kulaksiz R, Cebi C, Akcay E and Daskin A (2010). The protective effect of egg yolk from different avian species during the cryopreservation of Karayaka ram semen. Small Ruminant Research 88:12-15.

Mara L, Dattena M, Pilichi S, Sanna D, Branca A and Cappai P (2007). Effect of different diluents on goat semen fertility. Animal Reproduction Science 102(2):152-157.

Peris-Frau P, Sánchez-Ajofrín I, Martín Maestro A, Maside C, Medina-Chávez DA, García-Álvarez O, Fernández-Santos MDR, Montoro V, Garde JJ, Ramón M and Soler AJ (2021). Impact of cryopreservation on motile bubpopulations and tyrosine-phosphorylated regions of ram spermatozoa during capacitating conditions. Biology (Basel) 10(11):1213.

Sharafi M, Borghei-Rad SM, Hezavehei M, Shahverdi A and Benson JD (2022). Cryopreservation of semen in domestic animals: A review of current challenges, applications and prospective strategies. Animals 12:3271.

Sinanoglou VJ, Strati IF and Meimaroglou SM (2011). Lipid, fatty acid and carotenoid content of edible egg yolks from avian species: A comparative study. Food Chemistry 124:971-977.

Singh AP, Chakravarty AK and Mir MA (2018). Breeding performance of Murrah buffaloes in organised herd. Ruminant Science 7(2):207-212.

Su, L, Li X, Quan X, Yang S, Li Y, He X and Tang X (2008). A comparison of the protective action of added egg yolks from five avian species to the cryopreservation of bull sperm. Animal Reproduction Science 104:212-219.

Yadeta JF (2020). Review on Potential of Reproductive Technology to Improved Ruminant Production in Ethiopia. Journal of Biology, Agriculture and Healthcare 10(8):10-24.