25-Title: Comparative study of kappa-casein genetic variants and its association with lactose, snf and milk density traits in Sahiwal and HF cross bred of cow
Authors: Akhilesh Pandey, Yogita Pandey, MS Thakur, RK Vandre, Vaishali Khare and Amit Jha
Source: Ruminant Science (2023)-12(1):131-136.
How to cite this manuscript: Pandey Akhilesh, Pandey Yogita, Thakur MS, Vandre RK, Khare Vaishali and Jha Amit (2023). Comparative study of kappa-casein genetic variants and its association with lactose, SNF and milk density traits in Sahiwal and HF cross bred of cow. Ruminant Science 12(1):131-136.
Abstract
The present study revealed polymorphic variants and their association with milk production traits at the k-casein gene (CNS3) locus in Sahiwal and HF Crossbred cows. The frequency of ‘A’ allele was found to be higher as compared to ‘B’ allele in above both breeds of cattle under the study. Chi-square values for testing correspondence between observed and expected genotypic frequencies at this locus were found to be non-significant in the Sahiwal breed of cattle, indicating that the populations of these animals were in Hardy-Weinberg equilibrium at this locus, while the Chi-square value was found to be significant (p<0.05) for HF crossbred cattle revealing the deviation from Hardy Weinberg disequilibrium at this k-casein (CSN3) gene locus. Association of polymorphic variants of k-Casein (CSN3) gene with milk quality traits revealed that the AA and AB genotype of HF crossbred showed non- significantly but higher lactose (%) in milk than both AA genotype and AB genotypes of Sahiwal cow. Both AA and AB genotypes of Sahiwal and HF crossbred cattle also showed non-significant differences between them self. The AA genotyped animals in above both breeds of cattle revealed non-significant differences with AB genotype. The AB genotype of Sahiwal showed higher SNF (%) in the milk than both AA and AB genotypes of HF crossbred along with its own AA genotype. The milk density between AA and AB genotypes of Sahiwal and HF crossbred cattle showed a non-significant difference. The higher mean milk density (Kg/L) was noticed for AA and AB genotypes of HF crossbred as compared to AA and AB genotypes of Sahiwal.
References
Alphonsus C and Essien IC (2012). The relationship estimates amongst milk yield and milk composition characteristics of Bunaji and Friesian x Bunaji cows. African Journal of Biotechnology 11(36):8790-8793.
Alim MA, Dongxiao S, Yi Z, Yuan Z, Zhang Q and Lin L (2015). DNA polymorphisms in the beta-lactoglobulin and kappa–casein genes associated with milk production traits in dairy cattle. Bioresearch Communications 2(1):82-86
Awad A, El Araby IE, El-Bayomi KN and Zaglool AW (2016). Association of polymorphisms in kappa casein gene with milk traits in Holstein Friesian cattle. Japan Journal of Veterinary Research 64:39-43.
Bittante G, Penasa M and Cecchinato A (2012). Genetics and modelling of milk coagulation properties. Journal of Dairy Science 95:6843-6870.
Inamdar Basavraj, Naveen Kumar GS, Sudha KV and Yathish HM (2019). Detection of A1 and A2 alleles of beta-casein gene in Amruthmahal cattle. Ruminant Science 8(2):201-203.
John SW, Weitzner G, Rozen R and Scriver CR (1991). A rapid procedure for extracting genomic DNA from leukocytes. Nucleic Acid Research 19(2):408.
Kubkomawa HI (2017) Indigenous breeds of cattle, their productivity, economic and cultural values in Sub-Saharan Africa: A review. International Journal of Research Studies in Agricultural Sciences 3(1):27-43.
Caroli A, Chiatti F, Chessa S, Rignanese D, Bolla P and Pagnacco G (2006). Focusing on the goat casein complex. Journal of Dairy Science 89(8):3178-3187
Cosenza G, Pauciullo A, Colimoro L, Mancusi A, Rando A and Di Berardino D (2007). A SNP in the goat CSN2 promoter region is associated with the absence of beta-casein in milk. Animal Genetics 38(6):655-658.
Duifhuis-Rivera T, Lemus-Flores MÁ, Ayala-Valdovinos DR, Sánchez -Chiprés J, Galindo-García K, Mejía-Martínez E and González C (2014). Polymorphisms in beta and kappa casein are not associated with milk production in two highly technified populations of Holstein cattle in México. Journal of Animal and Plant Sciences 24(5):1316-132.
George Jilmil, Kuralkar SV, Ali SA, Bankar PS, Deshmukh SG and Kuralkar Prajakta (2018). Study of polymorphism of kappa casein gene in Marathwadi and Purnathadi buffalo. Ruminant Science 7(2):213-215.
Ghuge Pandurang, Yeotikar Pranesh, Ghorpade Prabhakar, Gaikwad Satish, Amrutkar Suhas, Kharwadkar Makrand and Pangaonkar Laxmikant (2022). Studies on assessment of ß-casein variants of Red Kandhari and crossbred cows of Marathwada region. Ruminant Science 11(2):339-342.
Molee A, Duanghaklang N, Na-Lampang P (2012). Effects of acyl-CoA: diacylglycerol acyl transferase 1 (DGAT1) gene on milk yield traits in crossbred Holstein dairy cattle. Tropical Animal Health 44 (4):751-755.
Neamt RI, Gheorghe S, Stelian A, Ludovic TC, Dinu G and Daniela EI (2017). The influence of CSN3 and LGB polymorphism on milk production and chemical composition in Romanian Simmental cattle. Acta Biochimica Polonica 64(3):493-497.
Ng-Kwai-Hang KF, Monardes HG and Hayes JF (1990). Association between genetic polymorphism of milk proteins and production traits during three lactations. Journal of Dairy Science 73:3414-3420.
Stevanovic M, Stanojcic S and Djurovic J (2000). Molecular marker technologies and selection for the traits of economic interest. Biotechnology in Animal Husbandry 16(1-2):25-34.
Rachagani S and Gupta ID (2008). Bovine kappa-casein gene polymorphism and its association with milk production traits. Genetics and Molecular Biology 31(4):893-897.
Dogru U (2015). Beta-Lactoglobulin genetic variants in Brown Swiss dairy cattle and their association with milk yield and quality traits. Journal of Animal and Plant Science 25(2):595-598.
Deb R, U. Singh S Kumar, Singh R, Sengar G and Sharma A (2014). Genetic polymorphism and association of kappa-casein gene with milk production traits among Frieswal (HF× Sahiwal) cross breed of Indian origin Iran. Journal of Veterinary Research 15:406-408.
Rachagani S and Gupta ID (2008). Bovine kappa-casein gene polymorphism and its association with milk production traits. Genetics and Molecular Biology 31:893-897.
Shah Ruksana M, Ganai NA, Shanaz S, Sheikh FD, Khan Nusrat N and Hamadani Ambreen (2021). Kappa-casein gene polymorphism and its association with milk production traits in dairy cattle of Kashmir, India. Ruminant Science 10(1):43-48.
Singh U, Deb R, Kumar S, Singh R, Sengar G and Sharma A (2014). Association of prolactin and beta-lactoglobulin genes with milk production traits and somatic cell count among Indian Frieswal (HF×Sahiwal) cows. Biomarkers and Geonomic Medicine 7(1):38-42.
Snedecor GW and Cochran WG (1994). Statistical Method. 8th Edn, Iowa State College Press, Iowa USA. p 950.
Stipp AT, Bignardi PR, Polifrederico RC, Sivieri K and Costa MK (2013). Polimorfismosgenéticosda kappa-caseína e dabetalactoglobuline e produção de leiteem bovinos. ArquivoBrasileiro de MedicinaVeterinária e Zootecnia 65(1):275-280.
Szymanowsky M, Eulalia S, Marek L and Lech Z (2004). Association of nucleotide-sequence polymorphism in the 5’-flanking regions of bovine casein genes with casein content in cow’s milk. INRAEDP Sciences 84:579-590.
Tamura K, Nei M and Kumar S (2004). Prospects for inferring very large phylogenies by using the neighbor-joining method. In: Proceedings of the National Academy of Sciences of the United States of America 101:11030-11035.
Threadgill DW and Womack JE (1990). Genomic analysis of the major bovine milk protein genes. Nucleic Acids Research 18:6935-6942.
Thompson JD, Higgins DG, Gibson TJ and Clustal W (1994). Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 22:4673-4680.
Yeh FC, Yang RC, Boyle TBJ, Ye ZH and Mao JX (1999). Popgene 32 version 1.32, the user-friendly shareware for population genetic analysis. Molecular Biology and Biotechnology Centre, University of Alberta, Canada.