18-Title: Molecular characterisation of estrogen receptor alpha gene and its association with reproductive and productive traits in Indian cattle breeds
Authors: Vishakha Uttam, Madhu Tiwari, Satyendra Pal Singh, Deepak Sharma and Avneesh Kumar
Source: Ruminant Science (2023)-12(2):279-284.

Abstract

How to cite this manuscript: Uttam Vishakha, Tiwari Madhu, Singh Satyendra Pal, Sharma Deepak and Kumar Avneesh (2023). Molecular characterisation of estrogen receptor alpha gene and its association with reproductive and productive traits in Indian cattle breeds. Ruminant Science 12(2):279-284.
Abstract
Function of the estrogen hormone is intimately correlated and regulated by the estrogen receptor alpha (ER-alpha) gene. Estrogen hormone influences the development and function of reproductive organs, such as the mammary gland, ovary, and uterus. In this current research, we cloned and characterized a partial coding sequence (CDS) of exon 1 from the ER-alhpa gene in Sahiwal and Hariana cattle breeds and study investigating polymorphisms was carried out in 5’ UTR of ER-alpha gene using BglI/PCR-RFLP assay. The nucleotide and amino acid sequences of the ER-alpha exon 1 in Sahiwal and Hariana cattle were highly analogous to those in exotic and crossbred cattle breeds. The PCR-RFLP assay uncovered three different genotype categories namely AA, AB, and BB where genotype AB was almost as frequent as BB genotype followed by AA genotype and the frequency of the B allele was higher than that of A allele in all the screened animals. Chi-square analysis indicated that the examined animal population adhered to the Hardy-Weinberg equilibrium. While an association study detected a notable significant (p<0.05) difference in lactation I where the BB genotype showed a higher lactation period than other genotypes in Hariana cattle. Future research, to identify the function of the cattle ER-alpha gene as a potential gene marker, will be strengthened by the information provided from our study. Our results should be further validated with a larger sample size that can be later implemented in the upgradation of the breeding programs of the studied cattle breeds. References Amenyogbe E, Chen G, Wang Z, Lu X, Lin M and Lin AY (2020). A review on sex steroid hormone estrogen receptors in mammals and fish. International Journal of Endocrinology. Falconer DS and Mackay TFC (1996). Genotypic frequencies. Introduction to Quantitative Genetics. Ed. 4. John Wiley and Sons, New York. Fu M, Xiong XR, Lan DL and Li J (2014). Molecular characterization and tissue distribution of estrogen receptor genes in domestic yak. Asian-Australasian Journal of Animal Sciences 27(12):1684. Jedrzejczak M, Grzesiak W, Szatkowska I, Dybus A, Muszynska M and Zaborski D (2011). Association between polymorphisms of CYP19, CYP21, and ER1 genes and milk production traits in Black-and-White cattle. Turkish Journal of Veterinary and Animal Sciences 35(1):41-49. Keskin A, Oner Y and Yilmazbas-Mecitoglu G (2015). Distributions of CYP19, ER-alpha and PGR allele frequencies between fertile and subfertile Holstein-Friesian heifers. KafkasUniv Vet Fak Derg 21:893-898. Khalil MH (2020). Molecular applications of candidate genes in genetic improvement programs in livestock. Egyptian Journal of Animal Production 57:1-23. Mohamadnejad-Sangdehi F, Rahimi-Mianji G, Safdari-Shahroudi M, Razavi-Sheshdeh SA, and Gholami M (2015). Distribution of allele frequencies at 52 -flanking region of CYP19 and ER-alpha genes between Iranian Simmental and three indigenous cattle breeds. Iranian Journal of Applied Animal Science 5(2):301-307. Paterni I, Granchi C, Katzenellenbogen JA and Minutolo F (2014). Estrogen receptors alpha (ER-alpha) and beta (ER-alpha): Subtype-selective ligands and clinical potential. Steroids 90:13-29. Sambrook J and Russell DW (2001). Molecular Cloning: A Laboratory Manual. Ed. 1, 112.Cold Spring Harbor Laboratory Press, New York, USA. Shah Ruksana M, Ganai NA, Shanaz S, Sheikh FD, Khan Nusrat N and Hamadani Ambreen (2021). Kappa-casein gene polymorphism and its association with milk production traits in dairy cattle of Kashmir, India. Ruminant Science 10(1):43-48. Sharma BL, Bhatt VK, Shukla MK, Thapak Swadesh, Gupta KK, Sharma Satendra and Parasar Rahul (2014). Periparturient hormonal profile and therapeutic management of cervicovaginal prolapse in murrah buffaloes. Ruminant Science 3(1):29-32. Szatkowska I, Grzesiak W, Jêdrzejczak M, Dybus A, Zaborski D and Jankowiak D (2011). An analysis of CYP19, CYP21 and ER genotypes in Polish Holstein-Friesian cows with regard to the selected reproductive traits. Acta Veterinaria Brno 80(1):65-71. Szreder T and Zwierzchowski L (2004a). Polymorphism within the bovine estrogen receptor-alpha gene 5'-region. Journal of Applied Genetics 45(2):225-236. Szreder T and Zwierzchowski L (2004b). RFLP-TspRI polymorphism within exon 1 of the bovine estrogen receptor-alpha (ER-alpha) gene. Animal Science Papers and Reports 22(4). Szreder T Zelazowska B, Zwierzchowski L and Pareek CS (2007). A novel nucleotide sequence polymorphism in the 52-noncoding region of bovine estrogen receptor alpha gene, the RFLP-Sna BI. Biochemical Genetics 45:255-262. Szreder T, Oprzadek J, Zelazowska B, Dymnicki E and Zwierzchowski L (2011). Polymorphism A/C in exon 7 of the bovine estrogen receptor alpha (ER alpha) gene and its association with functional and milk production traits in Red-and-White cattle. Animal Science Papers and Reports 29(4):281-291. Tamura K, Dudley J, Nei M and Kumar S (2007). MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Molecular Biology and Evolution 24(8):1596-1599. Tang KQ, Yang WC, Pai B, Li SJ, Chen L and Yang LG (2013). Effects of PGR and ESR-alpha genotypes on the pregnancy rates after embryo transfer in Luxi cattle. Molecular Biology Reports 40(1):579-584. Tiwari Uma Shanker, Shukla Satya Nidhi, Jain Anand Kumar, Shrivastava Shraddha, Mishra Ashutosh and Bisen Abhishek (2022). Effect of Murraya koenigii and Aegle marmelos leaf powder supplementation on physiobiochemical parameters in postpartum buffaloes. Ruminant Science 11(1):135-138. Vashi Y, Magotra A, Kalita D, Banik S, Sahoo NR, Gupta SK and Naskar S (2021). Evaluation of candidate genes related to litter traits in Indian pig breeds. Reproduction in Domestic Animals 56(4):577-585. Zahmatkesh A, Rahmani HR, Edriss MA and Sayed-Tabatabaei BE (2012). Estrogen receptor alpha (ER-alpha) gene and bovine performance: Is there any relation. Animal Science Papers and Reports 30(4):373-381. Zahmatkesh A, Rahmani HR, Edriss MA and Tabatabaei BES (2011). Bovine ER-alpha polymorphism in relation with production traits. International Conference on Food Engineering and Biotechnology IPCBEE 9.