28-Title: Effect of vitamin E on quality of Kankrej bull semen during cryopreservation
Authors: MU Prajapati, HC Nakhashi, KF Chaudhary and BN Suthar
Source: Ruminant Science (2024)-13(1):151-156.

Abstract

How to cite this manuscript: Prajapati MU, Nakhashi HC, Chaudhary KF and Suthar BN (2024). Effect of vitamin E on quality of Kankrej bull semen during cryopreservation. Ruminant Science 13(1):151-156.
Abstract
Total three Kankrej breeding bulls aged 3-4 years were used to collect 30 semen ejaculates (10 ejaculates from each bull) to determine the effect of different concentrations of Vitamin-E as a semen additive in Tris-egg yolk-based semen extender for cryopreservation. Each ejaculate was divided into three equal aliquots and diluted in a Tris-based extender containing Vitamin-E (1.5 mM and 2.0 mM as Group II and III; Group I as control) which were cooled to 5 ºC and frozen in 0.25 ml French mini straws using programmable bio freezer. Each group was evaluated for initial motility, sperm viability, sperm abnormality, acrosome integrity, Hypo-osmotic swelling test, seminal plasma enzymes (ALT and AST) and oxidative stress markers (SOD and LPO). Based on the findings of seminal characteristics, the leakage of AST and ALT enzymes and the oxidative stress markers (SOD activity and LPO levels) at post-equilibrated and post-thawed stages of semen cryopreservation, it was concluded that the addition of 1.5 mM vitamin-E in TFYG extender is more suitable to protect the Kankrej bull spermatozoa in comparison to 2 mM vitamin-E.
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