Title: Molecular characterization of Poonchi sheep

Authors: PM Azhar, D Chakraborty, D Kumar, Anamika, Tanveer A Sofi, N Kumar and RK Taggar

Source: Ruminant Science (2017)-6(2):231-236.

Cite this reference as: Azhar PM, Chakraborty D, Kumar D, Anamika, Sofi TA, Kumar N and Taggar RK (2017). Molecular characterization of Poonchi sheep. Ruminant Science 6(2):231-236.

Abstract

The present study on molecular characterization of Poonchi sheep was carried out on 30 unrelated Poonchi sheep by using six microsatellite markers namely MAF70, SPS113, OarFCB128, OarFCB48, BM1329 and MAF209. PCR amplifications were performed with some modifications and PCR products were run on 8% Urea-PAGE. Genotyping was done by visualizing the bands. Microsatellite data were analyzed by using molecular biology software program (POPGENE). A total of 31 alleles were observed by six microsatellite markers in Poonchi sheep. The Chi-square test revealed a non-significant effect of different genotypes for Hardy-Weinberg Equilibrium. The mean observed (na) and expected (ne) number of alleles in the population were 5.17±0.75 and 3.98±0.58, respectively. The Shannon’s information index (I) were 1.25, 1.42, 1.51, 1.54, 1.66 and 1.45, respectively for MAF70, SPS113, OarFCB128, OarFCB48, BM1329 and MAF209 markers. The average expected heterozygosity ranged from 0.68 (MAF70) to 0.79 (BM1329). A broad variation was found in FIS value among different loci ranging from -0.22 (MAF90) to 0.50 (OarFCB128). Out of 31 alleles observed in the Poonchi sheep population 22 alleles showed negative FIS values. In the Ewens-Watterson Test for neutrality of microsatellite loci the mean value was ranged from 0.28±0.01 (BM1329) to 0.42±0.01 (MAF70). From the present study it can be concluded that microsatellite markers can be successfully used for genetic characterization of Poonchi sheep with substantial genetic variation and all the loci under study were (100%)  polymorphic in Poonchi sheep.

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