Title: Effect of leptin gene polymorphism on reproduction and production traits in Sahiwal cattle

Authors: V Pandey, R Nigam, SP Singh, Deepak Sharma, Madhu Tiwari and Rambachan

Source: Ruminant Science (2017)-6(2):237-242.

Cite this reference as: Pandey V, Nigam R, Singh SP, Sharma Deepak, Tiwari Madhu and Rambachan (2017). Effect of leptin gene polymorphism on reproduction and production traits in Sahiwal cattle. Ruminant Science 6(2):237-242.

Abstract

This study was designed to explore the genetic polymorphism in LEP gene by LEP/BsaAI assay in Sahiwal cattle and their associations with reproduction and production traits. The blood samples were collected from 69 Sahiwal cattle and LEP/BsaAI genotypes were analyzed using PCR-RFLP method. The results showed three genotypes AA, AB and BB with 21.74, 50.72 and 27.54% frequencies, respectively and allele frequencies were 0.47 for A and 0.53 for B alleles. The results of the study revealed significant association of LEP/BsaAI genotypes with lactation period, total milk yield, milk yield in 300 days, peak yield and days to reach peak yield in Sahiwal cattle. This genetic information of bovine leptin gene could be valuable in marker assisted selection to improve milk production and reproductive potential of Sahiwal cattle.

References

Almeida SEM, Almeida EA, Moraes JCF and Weimer TA (2003). Molecular markers in the LEP gene and reproductive performance of beef cattle. Journal of Animal Breeding and Genetics 12:106-113.

Aslaminejad AA, Nassiry MR, Farajollahi H, Mahdavi M, Abbasi H and Javadmanesh A (2010). Polymorphism in Exon 3 of Leptin Gene in Iranian Native Cattle Breeds. Journal of Applied Animal Research 37:159-162.

Azari MA, Hasani S, Heidari M and Yousefi S (2012). Genetic polymorphism of leptin gene using PCR-RFLP method in three different populations. Slovak Journal of Animal Science 45(2):39-42.

Chebel RC and Santos JEP (2011). Association between leptin single nucleotide polymorphism and reproductive performance of lactating Holstein cows. Animal Reproduction Science 127:126-134.

Choudhary V, Kumar P, Bhattacharya TK, Bhushan B and Sharma A (2005). DNA polymorphism of leptin gene in Bos indicus and Bos taurus cattle. Genetics and Molecular Biology 28(4):23-27.

Clempson AM, Pollott GE, Brickell JS, Bourne NE, Munce N and Wathes DC (2011). Evidence that leptin genotype is associated with fertility, growth, and milk production in Holstein cows. Journal of Dairy Science 94:3618-3628.

Dandapat A, Kumar D, Ghosh AK and Banerjee D (2009). Association of leptin gene polymorphism with growth, milk production and reproduction traits in Sahiwal and crossbred cattle. Indian Journal of Animal Science 79(9):892-896.

Datta S, Verma A, Gupta ID, Chauhan I, Azad MS, Yathish HM, Batul NK and Har T (2012). Identification of polymorphism in leptin gene of murrah buffalo by PCR-RFLP. Exploratory Animal and Medical Research 2:7-17.

Deshpande M, Rank DN, Vataliya PH and Joshi CG (2014). Study of leptin gene polymorphism in Mehsana buffaloes (Bubalus bubalis). Buffalo Bulletin 33(1):55-59.

Dubey PK, Goyal S, Yadav AK, Sahoo BR, Kumari N, Mishra SK, Niranjan SK, Arora R, Mukesh M and Kataria RS (2014). Genetic diversity analysis of the thyroglobulin gene promoter in buffalo and other bovines. Livestock Science 167:65-72.

Forhead AJ and Fowden AL (2009). The hungry fetus? Role of leptin as a nutritional signal before birth. Journal of Physiology 587:1145-1152.

Glantz M, Mansson HL, Stalhammar H and Paulsson M (2012). Effect of polymorphism in the leptin, leptin receptor and acyl-CoA:diacylglycerol acyltransferase 1 (DGAT1) genes and genetic polymorphism of milk proteins on bovine milk composition. Journal of Dairy Research 79:110-118.

Kadlecova V, Nemeckova D, Jecminkova K and Stadnik L (2014). Association of bovine DGAT1 and leptin genes polymorphism with milk production traits and energy balance indicators in primiparous Holstein cows. Mljekarstvo 64(1):19-26.

Komisarek J and Antkowiak I (2007). The relationship between leptin gene polymorphisms and reproductive traits in Jersey cows. Polish Journal of Veterinary Science 10:193-197.

Liefers SC, Veerkamp RF, Te Pas MFW, Delavaud C, Chilliard Y, Platje M and Vander LT (2005). Leptin promoter mutations affect leptin levels and performance traits in dairy cows. Animal Genetics 36(2):111-118.

Lien S, Undvold H, Lungland H and Vage DI (1997). Two novel polymorphisms in the bovine obesity gene (OBS). Animal Genetics 28:45-48.

Lindersoon M, Andersson-Eklund L, Koning DJ, Lunden A, Maki-Tanila A and Andersson L (1998). Mapping of serum amylase-1 and quantitative trait loci for milk production traits to cattle chromosome 4. Journal of Dairy Science 81:1454-1461.

Moravcikova N, Trakovicka A and Kasarda R (2012). Polymorphism within the intron region of the bovine leptin gene in Slovak Pinzgau cattle. Animal Science and Biotechnology 245(1):112-115.

Ngu NT, Quynh LTB, Hon NV, Nhan NTH, Khoa DVA, Hung LT and Xuan NH (2015). Influence of leptin genotypes on milk fat and protein content of cross bred Holstein friesian x laisind cows. Journal of Animal and Plant Science 25(1):304-308.

Pandey Vijay, Nigam R, Rambachan, Singh P, Singh SP and Madan AK (2016). Plasma leptin and biochemical profile around parturition in primiparous Sahiwal cows. Ruminant Science 5(2):227-233.

Pomp D, Zou T, Clutter AC and Barendse W (1997). Mapping of leptin to bovine chromosome 4 by linkage analysis of a PCR based polymorphism. Journal of Animal Science 75:1427.

Rambachan, Nigam R, Pandey V, Singh P, Singh SP and Sharma D (2016). Genetic polymorphism of leptin gene in relation with reproduction traits in Harian cows. Journal of Animal Research 7(3):425-429.

Rezaei M, Mirhosseini SZ and Hossein-Zadeh NG (2015). PCR-RFLP analysis of leptin gene and its association with milk traits in native cattle of Iran (Bos indicus). Revista Mexicana de Ciencias Pecuarias 6(1):15-24.

Sambrook J and Russel DW (2001). Molecular Cloning: A Laboratory Manual. 3rd Edn, Vol 1, Cold Spring Harbor Laboratory Press, New York, USA.

Souza FR, Mercadante ME, Fonseca LF, Ferreira LM, Regatieri IC, Ayres DR, Tonhati H, Silva SL, Razook AG and Albuquerque LG (2010). Assessment of DGAT1 and LEP gene polymorphisms in three Nelore (Bos indicus) lines selected for growth and their relationship with growth and carcass traits. Journal of Animal Science 88:435-441.

Trakovicka A, Moravcikova N and Kasarda R (2013). Genetic polymorphisms of leptin and leptin receptor genes in relation with production and reproduction traits in cattle. Acta Biochimica Polonica 60(4):783-787.