Identification of SNP in growth hormone gene and their association

Identification of SNP in growth hormone gene and their association with growth traits in Kenguri sheep

Title: Identification of SNP in growth hormone gene and their association with growth traits in Kenguri sheep

Authors: Vaishali Hiremath, MM Appannavar, Yathish HM, Sangamesh, SM Kartikesh, Vivek R Kasaralikar and Siddalingaswamy Hiremath

Source: Ruminant Science (2017)-6(2):221-224.

Cite this reference as: Hiremath Vaishali, Appannavar MM, HM Yathish, Sangamesh, Kartikesh SM, Kasaralikar VR and Hiremath Siddalingaswamy (2017). Identification of SNP in growth hormone gene and their association with growth traits in Kenguri sheep. Ruminant Science 6(2):221-224.

Abstract

The research was performed with an aim of identifying SNPs in growth hormone (GH) gene using PCR-RFLP analysis and to associate them with various growth traits in Kenguri sheep. For this, a fragment of 422bp spanning between exon 2 and 3 of GH gene was amplified from 60 Kenguri sheep. GH/ HaeIII RFLP analysis of these fragments for locus A781G has showed only AA (with 366 bp and 56bp bands) and AB (with 422bp, 366bp and 56 bp bands) genotypes and BB genotype was absent in the studied sheep population. The respective frequency of AA, and AB genotype was 0.42 and 0.58 and that of A and B allele was 0.71 and 0.29. Chi-square analysis of GH/HaeIII polymorphism showed noncompliance of Hardy Weinberg equilibrium in studied Kenguri population. The Mean±SE values for body weight (kg), height at wither (cm), body length (cm) and chest girth (cm) were 34.98±0.59, 70.97±0.375, 69.22±0.50 and 79.72±0.72 in ewes, while in rams the respective values were 37.46±0.69, 71.25±0.59, 69.18±0.90 and 83.18±0.84. Analysis of these data using ‘t’ test revealed a significant (p<0.05) difference between ram and ewe with respect to body weight and chest girth only. Association analysis revealed non-significant difference between AA and AB genotypes for body weight, height at wither, body length and chest girth both in rams and ewes of Kenguri sheep. The non correlation between different genotypes and growth traits in this investigation may be accounted to small sample size. However, the association may be established if the sample size is large and in turn it can be used for selection of animals for breeding to bring improvement in mutton production.

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