Title: EFFECT OF SEASON ON CERTAIN SEMINAL PLASMA ENZYMES PROFILE AT PRE-FREEZE AND POST THAW PERIOD IN THE HARYANA BULL
Authors: Vipin Singh, Anuj Kumar, CL Yadava, Akhil Patel, Brijesh Yadav, Vijay Panday and Atul Saxena
Source: Ruminant Science (2018)-7(2):279-282.
Cite this reference as: Singh Vipin, Kumar Anuj, Yadava CL, Patel Akhil, Yadav Brijesh, Panday Vijay and Saxena Atul (2018). Effect of season on certain seminal plasma enzymes profile at pre-freeze and post thaw period in the Haryana bull. Ruminant Science 7(2):279-282.
Abstract
The effect of seasonal variations on semen biochemical indices was studied in Haryana bull. Four fertile, healthy adult Haryana bulls, aged between 5.5-6.5 years and weighing between 450-550 kg, were used over a period of four months, divided into two seasons as winter season (December-January) summer season (May-June) for semen collection. Twenty-four semen samples (n=6/bull) were collected in each season by using artificial vagina. Each ejaculate was divided into two aliquots; one was assessed for physico-morphological and functional attributes viz., volume (ml), mass activity (0-5 scale), pH, sperm concentration (million/ml), progressive motility (%), sperm livability (%), HOST (%), total morphological sperm abnormality (%) and acrosomal integrity (%) were estimated at different stages of cryopreservation in different seasons., while other was used for evaluation of seminal biochemical parameters viz., superoxide dismutase (SOD), lipid peroxidation (LPO), glutathione-S-transferase (GST), and enzymatic glutamate oxaloacetate transaminase (GOT), and lactate-dehydrogenase (LDH) enzymes. profile. Semen SOD and GST concentration was significantly higher (p<0.05) during the winter season.
References
Aitken RJ, Buckingham D and Harkiss D (1993). Use of a xanthine oxidasefree radical generating system to investigate the cytotoxic effects ofreactive oxygen species on human spermatozoa. Journal of Reproduction and fertility 97:441-450
Alvarez JG and Storey BT (1992). Evidence for increased lipid peroxidative damage and loss of superoxide dismutase activity as a mode of sublethal cryodamage to human sperm during cryopreservation. Journal of Andrology 13:232-240.
Atig F, Raffa M, Ali HB, Abdelhamid K, Saad A and Ajina M (2012).Altered antioxidant status and increased lipid per-oxidation in semi-nal plasma of Tunisian infertile men. Indian Journal of Biological Science 8:139-149
Bilodeau J, Chatterjee S, Sirard M and Gagnon C (2000). Levels of antiox-idant defenses are decreased in bovine spermatozoa after a cycle offreezing and thawing. Molecular Reproduction and Development 55:282-288.
Brown DV, Senger PL, Stone SL, Froseth JA and Becker WC (1977). Glutathione peroxidase in bovine semen. Journal of Reproduction and fertility 50:117-118
Burgos C, Maldonado C, Gerez de Burgos NM, Aoki A and Blanco A (1995). Intracellular localization of the testicular and sperm-specific lactate deydrogenase isoenzyme C4 in mice. Biology of Reproduction 53:84-92
Dhami AJ, Panchal MJ and Kodagali SB (1990). Comparative biochemical and enzymatic study of Jersey, crossbred and Surti buffalo semen with special references to freezability and fertility. Indian Journal of Animal Research 24:1-8.
Habig WH, Pabst MJ and Jakoby WB (1974). Glutathione S-transferases. The first enzymatic step in mercupturic acid formation. Journal of Biochemistry 249:7130-7139.
Hammerstedt RH (1993). Maintenance of bioenergetic balance in sperm and prevention of lipid peroxidation: A review of the effect on design of storage preservation systems. Journal of Reproduction and fertility 5:675-690.
Iqbal M, Samad H A, Yaqub M and Nagib-ur-Rehman. (1984). Comparative study of cholesterol and total lipid in semen of Nili-Ravi Buffalo bulls and Sahiwal bulls. Pakistan Veterinary Journal 4:158-160.
Li TK (1975). The glutathione and thiol content of mammalian spermato-zoa and seminal plasma. Biological Reproduction 12:641-646.
Madesh M and Balasubramanian KA (1997). Micro titer plate assay for superoxide dismutase during MTT reduction by superoxide. Indian Journal of Biochem and Biophysics 35:184-188.
Mahawar Manish, Jain AK and Kumar Puneet (2012). Effect of environmental conditions on the activities of aminotransferases and lactate dehydrogenase in goats. Ruminant Science 1(1):73-76.
Mann T and Lutwak-Mann C (1981). Male reproductive function and semen. Spriger-Verlag. Berlin Heidelberg. NewYork 212-216.
Mandal DK, Nagpal PK and Gupta AK (2000). Seasonal variation in seminal attributes and sexual behaviour of Murrah buffalo bulls. Indian Journal of Dairy Science 53:278-283.
Mustapha AR, Beigh YA, Abhishek K, Rahul K, Omer D, Kumar A, Prasad JK, Bhure S, Srivastava N and Ghosh SK (2017). Effects of partial deoxygenation of extender on plasma membrane integrity and enzyme activity in frozen-thawed crossbred bull semen. Ruminant Science 6(2):327-331.
Ohkawa H, Ohishi N and Yagi K (1979). Assay of lipid peroxides in animal tissues by thiobarbituric acid reaction. Anaytical Biochemistry 95:351-358.
Parkinson TJ (1985). Seasonal variation in semen quality of bulls and correlations with metabolic and endocrine parameters. Veterinary Record 117:303-307.
Patel BR and Siddiquee GM (2013). Effect of semen additives on leakage of certain enzymes during cryopreservation of kankrej semen. Ruminant Science 2(1):63-66.
Quereshi MS, Samad HA, Khan Z and Hussain S (1995). Environmental effects on the quality of bovine semen. Thailand Journal of Veterinary Medicine 25:75-80
Rajoriya JS, Prasad JK, Ghosh SK, Kumar A, Perumal M and Kumar S (2013). Effect of season on enzymatic changes and cholesterol efflux in relation to freezability of Tharparkar bull semen. Assian pacific journal of Reproduction 2(4):280-288.
Singh V, Kumar A, Yadav CL, Patel A, Yadav V and Saxena A (2018). Effect of Season on Physico-Morphological Attributes of Cryopreserved Hariana Bull Spermatozoa. Indian Journal of Veterinary Sciences and Biotechnology 14(1): 47-51.
Sirat MP, Sinha AK, Singh BK and Prasad RL (1996). Effects of cryoprotectants on release of various enzymes from buck spermatozoa during freezing. Theriogenology 45:405-416.