Title: GENETIC POLYMORPHISM OF PGLYRP-1 GENE AND ITS ASSOCIATION WITH MASTITIS IN DEONI AND HOLSTEIN FRIESIAN CROSSBRED COWS
Authors: Shivashanker, MR Jayashankar, R Nagaraja, S Yathiraj, YB Rajeshwari and S Isloor
Source: Ruminant Science (2018)-7(2):199-202.
Cite this reference as: Shivashanker, Jayashankar MR, Nagaraja R, Yathiraj S, Rajeshwari YB and Isloor S (2018). Genetic polymorphism of PGLYRP-1 gene and its association with mastitis in Deoni and Holstein Friesian crossbred cows. Ruminant Science 7(2):199-202.
Abstract
The present study was conducted to determine the polymorphism of PGLYRP-1 gene and its association with mastitis in Deoni and HF crossbred cattle. Milk and blood samples were collected from 244 animals comprising of 152 HF crossbred and 92 Deoni cows. The milk samples were screened for subclinical mastitis using three indirect diagnostic tests viz., California Mastitis Test (CMT), Electrical Conductivity (EC) and Somatic Cell Count (SCC). However, the animals were classified into mastitis free and mastitis affected groups based on SCC alone because of its higher sensitivity and specificity. Miller’ high salt method was followed to isolate genomic DNA from venous blood. The quantity and quality of DNA was ascertained by spectrophotometer and 0.8 per cent agarose gel electrophoresis. A 796 bp of exon1 of PGLYRP 1 gene was amplified by PCR using published primers. The PCR-RFLP analysis with ApaI restriction enzyme revealed three genotypes, viz., GG, GC and CC in HF crossbred cattle with frequency of 0.22, 0.65 and 0.13 percent, respectively. In Deoni cattle, only two genotypes, GG and GC, were observed with frequency of 0.88 and 0.12 percent, respectively. The Chi-square analysis revealed a significant association of GC genotype with occurrence of mastitis in H.F. crossbred cattle.
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