Title: EXPRESSION OF Mn-SOD AND Cu, Zn-SOD mRNA IN RELATION TO THERMOTOLERANCE OF SAHIWAL AND KARAN-FRIES CATTLE
Authors: P Mayengbam*, TC Tolenkhomba and RC Upadhyay
Source: Ruminant Science (2017)-6(1):69-74.
Cite this reference as: Mayengbam P, Tolenkhomba TC and Upadhyay RC (2017). Expression of Mn-SOD and Cu, Zn-SOD mRNA in relation to thermotolerance of Sahiwal and Karan-Fries cattle. Ruminant Science 6(1):69-74.
Abstract
The present study aimed to investigate the expression of Mn-SOD and Cu, Zn-SOD mRNA in relation to physiological index of Sahiwal and Karan-Fries. Six numbers each of Sahiwal and Karan-Fries heifers in the age group of 2 to 2.5 years were exposed at 45±1oC and 50% relative humidity (RH) inside a psychrometric chamber for 4 h. Respiration rate (RR), heart rate (HR) and rectal temperature (RT) were recorded to calculate Dairy Search Index (DSI). Blood samples collected at 0, 1, 2, 3 and 4 h of heat exposure were used for isolation of lymphocytes and subsequent estimation of Mn-SOD and Cu, Zn-SOD mRNA expression by RT-PCR. RR and RT increased after 1 h in both the breeds while HR was not affected by heat exposure. Heat exposure increased DSI of Sahiwal and Karan-Fries after 2 and 1 h, respectively. Expression of Mn-SOD and Cu, Zn-SOD mRNA of both the breeds were not influenced by heat exposure. The two breeds exhibited similar RR, RT, HR, DSI, Mn-SOD and Cu, Zn-SOD mRNA expression during heat exposure.
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