Title: Comparative efficacy of enrichment of spermatozoa using swim up vis-à-vis nano-technique in buffalo bull

Authors: Omer Din, AR Mustapha, Beigh Yaqoob Amin, K Rahul, Abhishek K, SK Ghosh, JK Prasad, Ajay Kumar, Praveen Singh, S Bag, Megha Pande and N Srivastava

Source: Ruminant Science (2018)-7(1):73-76.

Cite this reference as: Din Omer, Mustapha AR, Amin Beigh Yaqoob, Rahul K, Abhishek K, Ghosh SK, Prasad JK, Kumar Ajay, Singh Praveen, Bag S, Pande Megha and Srivastava N (2018). Comparative efficacy of enrichment of spermatozoa using swim up vis-à-vis nano-technique in buffalo bull. Ruminant Science 7(1):73-76.

Abstract

Comparative efficacy of depletion of dead/damaged spermatozoa using nano-purification and swim up technique was studied. Semen ejaculate of one Murrah buffalo bull was divided into three aliquots, first one was processed following routine protocol (Gr I), whereas second and third aliquot were subjected to swim up and nano-purification protocols for removal of dead spermatozoa. Though there was a significant decrease in the sperm concentration (307.28±4.90, 287.35±3.25 and 266.68±5.25 million/ mL, in Gr I, II, and III, respectively), a significant improvement in viability (76.21±1.07, 73.44±3.17, and 87.85±0.80%, respectively in Gr I, II and III) in reverse order was recorded. The mean efficiency (per cent) of nano-purification based on concentration, viability, individual progressive motility, and sperm morphological abnormalities was 13.73±0.94, 11.63±0.70, 11.67±1.67, and 3.53±0.52 at lag-stage (at the end of nano-purification), respectively. Results from present study revealed nano-purification using anti-ubiquitin particles effectively removed dead/damaged spermatozoa at lag-stage much more efficiently than swim up technique.

 

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