1-Title: Isolation and characterization of two lytic brucellaphages from Jammu

Authors: S Gazal, P Kaur, D Narang, HM Saxena and TS Rai

Source: Ruminant Science (2023)-12(1):1-7.

How to cite this manuscript: Gazal S, Kaur P, Narang D, Saxena HM and Rai TS (2023). Isolation and characterization of two lytic brucellaphages from Jammu. Ruminant Science 12(1):1-7.

Abstract

Bovine brucellosis is endemic in India and causes huge economic losses to dairy farmers. Brucellaphages can be used to control brucellosis. We have isolated two brucellaphages, BpJ1 and BpJ2 from sewage samples of dairy farms in Jammu. Both phages lysed Brucella abortus strain 19, B. abortus strain 99 and 10 field isolates of B. abortus maintained in the Department of Veterinary Microbiology, GADVASU, Ludhiana but did not lyse any heterologous bacteria viz. Staphylococcus aureus, Streptococcus agalactiae, Salmonella species, Escherichia coli and Pseudomonas aeruginosa. Lytic plaques for both phages were circular having diameter 0.5- 2.0 mm. The titre of BpJ1 was 3.8×106 pfu per ml and titre of BpJ2 was 4.2×106 pfu per ml at a phage concentration of 10-4. On Electron Microscopy, both phages were found to be tailed and belonged to order Caudovirales family Siphoviridae. BpJ1 was completely inactivated at 55 ºC after 1 hr, at -20 ºC after 4 hr; after 4 hr in sunlight; at 4 mins in UV light; at 1 hr at pH 2, after 4 hr at pH 4, after 15 mins on exposure to 10% Chloroform; at 15 mins of exposure to 1% SDS; at 60 mins of exposure to Lysozyme and at 1 hr on exposure to Proteinase K. BpJ2 was completely inactivated at 55 ºC after 1 hr, at -20 ºC after 4 hr; after 4 hr in sunlight; at 6 mins in UV light; at 4 hr at pH 2, after 4 hr at pH 4; after 15 mins on exposure to 10% Chloroform; after 15 mins on exposure to 1% SDS; at 1 hr on exposure to Lysozyme and at 1 hr on exposure to Proteinase K.

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