22-Title: Carrier frequency of fxid genetic disorder in Sahiwal and Holstein Friesian cattle in Chhattisgarh, India

Authors: Vineet Kumar, Asit Jain, Tripti Jain, Kishore Mukherjee, Aakanksha Rathore, Kaiser Parveen, Deepti K Barwa, Mohan Singh, Farheen Ansari and Sita Prasad Tiwari

Source: Ruminant Science (2023)-12(1):117-120.

How to cite this manuscript: Kumar Vineet, Jain Asit, Jain Tripti, Mukherjee Kishore, Rathore Aakanksha, Parveen Kaiser, Barwa Deepti K, Singh Mohan, Ansari Farheen and Tiwari Sita Prasad (2023). Carrier frequency of fxid genetic disorder in Sahiwal and Holstein Friesian cattle in Chhattisgarh, India. Ruminant Science 12(1):117-120.

Abstract

Numerous autosomal and sex-linked recessive disorders have detrimental economic impacts and decrease the rate of genetic improvement. In the present study, genetic screening of Sahiwal and Holstein Friesian animals was conducted for factor XI deficiency (FXID) genetic disorder, an autosomal recessive genetic defect, using PCR and sequencing techniques. Genomic DNA was extracted from 50 Sahiwal and 100 Holstein Friesian animals using commercially available kits. The quality and quantity of genomic DNA were determined using agarose gel electrophoresis and spectrophotometer, respectively. Gene-specific primer was used for in-vitro amplification of the FXI gene. A single specific band of 244 bp in size was visualized. The PCR product was purified and sent for sequencing to confirm the mutation in the targeted DNA sequence. Affected or carrier animals for FXID disorder were not detected in the selected animals. Thus, gene and genotype frequencies of FXID carriers were zero and the frequency of normal animals was found to be 100 per cent. It is therefore strongly advised that the implementation of screening programmes for a large number of animals used for breeding would help in decreasing the prevalence of this genetic disorder in the dairy farms.

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