5-Title: Genetic variation in intron 2 region of myostatin gene in Marwari sheep of Rajasthan

Authors: Govind Singh Dhakad, GC Gahlot, Navav Singh, PC Sharma, Manoj Kumar and Rakesh Kumar

Source: Ruminant Science (2022)-11(1):25-28.

How to cite this manuscript: Dhakad Govind Singh, Gahlot GC, Singh Navav, Sharma PC, Kumar Manoj and Kumar Rakesh (2022). Genetic variation in intron 2 region of myostatin gene in Marwari sheep of Rajasthan. Ruminant Science 11(1):25-28.

Abstract

The economic success of small ruminant based livestock production system depends on the factors affecting the muscular growth. Myostatin (MSTN), a negative regulator of skeletal muscle development in mammals, represents a key target for genetic investigations in meat-producing animals, with mutations responsible for increased skeletal-muscle mass, which was described in several livestock species. The present study aimed to investigate the genetic variation in the intron 2 region of the myostatin gene in Marwari lambs through single nucleotide polymorphisms (SNP’s) based markers. Blood samples were collected from randomly selected Marwari sheep ((n=71), and DNA was extracted using the spin column method. A 311bp fragment comprising the intron 2 region of the MSTN gene was amplified through PCR using designed homologous primers (GenBank accession number JN856480) at an annealing temperature of 54°C. Initial screening of collected specimens was carried out through single stranded conformation polymorphism (SSCP) and showed two different conformations (AA and AB) in the intron 2 region of the MSTN gene in Marwari sheep. The frequencies of “AA” and “AB” genotypes were observed to be 0.86 (n=61) and 0.14 (n=10), respectively. Sequencing and clustal analysis was carried out to detect SNP’s responsible for genetic variation among individuals. The two conformational patterns detected were observed to be the result of three different nucleotides substitution in the amplified fragment.

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