50-Title: Isolation and antibiotic sensitivity profiling of Escherichia coli and Klebsiella spp. isolates from pneumonic lungs of sheep and goats

Authors: Vikas Galav, Alka Galav and Sandeep Kumar Sharma

Source: Ruminant Science (2022)-11(1):243-250.

How to cite this manuscript: Galav Vikas, Galav Alka and Sharma Sandeep Kumar (2022). Isolation and antibiotic sensitivity profiling of Escherichia coli and Klebsiella spp. isolates from pneumonic lungs of sheep and goats. Ruminant Science 11(1):243-250.

Abstract

Respiratory tract infections are major health deterrent for production among the small ruminants of Rajasthan. A comparative study in sheep and goats was undertaken to investigate the etiopathology and antibiogram dynamics of two important species of Enterobacteriaceae in respiratory tract infections, which are also categorized under colonizing opportunistic pathogen (COP).  Lung samples with visible pneumonic gross lesions were collected during necropsy of sheep and goats and were further subjected to the histopathological investigation; lungs with bronchopneumonia or interstitial pneumonia were identified. The bacterial swabs from 25 such pneumonic lungs, each from sheep and goats yielded a total 12 E. coli (7 from sheep and 5 from goats) and 7 Klebsiella spp. (3 from sheep and 4 from goat) isolates.  All E. coli and Klebsiella spp. isolates showed typical culture and biochemical characteristics. The antibiogram profiling revealed multidrug resistance. The E. coli isolates from sheep and goats showed complete resistance against 16 and 10 commonly used antibiotics respectively, whereas, the Klebsiella spp. of sheep and goat origin were resistant to 20 and 10 antibiotics, respectively. The striking difference between the isolates of sheep and goats origin was difference in their resistance pattern; both bacterial types from goats were more sensitive to antibiotics as compared to sheep. However, all isolates were resistant to the important antibiotics used for the treatment of human infection like Rifampicin.  The drug Imipenem was observed to be the most suitable antibiotic against all Klebsiella spp. isolates and EXPEC isolate both of sheep or goat origin were found sensitive to Imipenem. The complex interplay among human, animal and environment is contributing to the silent spread of antibiotic resistance genes through colonizing opportunistic pathogens, which requires urgent attention to prevent a public health crisis.

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