32-Title: Efficacy of epididymal spermatozoa for in vitro fertilization in caprine species

Authors: A Suresh, MK Shukla, Dharmendra Kumar, OP Shrivastava and Neeraj Verma

Source: Ruminant Science (2020)-9(2):383-386.

How to cite this manuscript: Suresh A, Shukla MK, Kumar Dharmendra, Shrivastava OP and Verma Neeraj (2020). Efficacy of epididymal spermatozoa for in vitro fertilization in caprine species. Ruminant Science 9(2):383-386.

Abstract

The fertilization capacity of caprine epididymal spermatozoa in an in vitro embryo production system was evaluated in this study. Oocytes and spermatozoa were recovered from ovaries and epididymis, respectively, procured from a local small animal abattoir. The abattoir derived cumulus oocyte complexes (COCs) were subjected to in vitro maturation and in vitro fertilization with epididymal spermatozoa harvested from the epididymis of slaughtered animals collected within 2-3 hours of slaughter and stored at 4oC up to 24 hours. Cytoplasmic maturation, cleavage and blastocyst rates of 89.78±0.50, 63.88±2.33 and 25.38±2.08%, respectively were recorded for in vitro embryo production with abattoir derived male and female gametes. In conclusion, the use of abattoir derived spermatozoa for in vitro embryo production can be useful for the conservation of elite germplasm from dead animals.

References

Al-Timimi IHS (2013). Using of epididymal sperms of local Iraqi bucks for in vitro fertilization. Al-Qadisiyah Journal of Veterinary Medicine Sciences 12(2):126.

Ball GD, Leibfried ML, Lenz RW, Ax RL, Bavister BD and First NL (1983). Factors affecting successful in vitro fertilization of bovine follicular oocytes. Biology of Reproduction 28(3):717-725.

Blash S, Melican D and Gavin W (2000). Cryopreservation of epididymal sperm obtained at necropsy from goats. Theriogenology 54(6):899-905.

Cognié Y, Poulin N, Locatelli Y and Mermillod P (2004). State-of-the-art production, conservation and transfer of in-vitro-produced embryos in small ruminants. Reproduction, Fertility and Development 16(4):437-445.

Guerrero CA, Gentry G, Saenz J, Bondioli KR and Godke RA (2009). Birth of calves after artificial insemination with cryopreserved bovine epididymal spermatozoa harvested from postmortem bulls. Reproduction, Fertility and Development 21(1):105.

Kumar D, Jain SK and Sarkhel BC (2016). A comparison of in vitro fertilization potential of sperm obtained from refrigerated (4°C) epididymis and fresh ejaculation of buck under different embryo culture media. Indian Journal of Animal Sciences 86(8):883-886.

Paramio MT and Izquierdo D (2016). Recent advances in vitro embryo production in small ruminants. Theriogenology 86(1):152-159.

Pavlok A, Torner H, Motlik J, Fulka J, Kauffold P and Duschinski U (1988). Fertilization of bovine oocytes in vitro: Effect of different sources of gametes on fertilization rate and frequency of fertilization anomalies. Animal Reproduction Science 16(3-4):207-213.

Singh AP, Kumar D, Gopalakrishna R, Ranjan R, Pandey SK and Sarkhel BC (2016). Comparison of culture media for their effects on development of caprine IVF embryos using fresh and cryopreserved semen. Indian Journal of Animal Research 50(6):846-850.

Suresh A, Verma N, Kumar D, Shukla MK and Shrivastava OP (2018). Simulated physiological oocyte maturation augments the developmental competence of in vitro produced goat embryos. In Proceedings of XXXIV Annual Convention and International Symposium of ISSAR on ‘Productivity enhancement through augmenting reproductive efficiency of livestock for sustainable rural economy’, 28-30th December 2018, Anand. pp 63.

Zaraza J, Oropeza A, Velazquez MA, Korsawe K, Herrmann D, Carnwath JW and Niemann H (2010). Developmental competence and mRNA expression of preimplantation in vitro-produced embryos from prepubertal and postpubertal cattle and their relationship with apoptosis after intraovarian administration of IGF-1. Theriogenology 74(1):75-89.